DOT · NON-DOT · CONSTRUCTION EMPLOYER TESTING
Mobile Drug & Alcohol Testing in Charlotte, North Carolina
On-site DOT-compliant collections at Charlotte Douglas International Airport for FAA-regulated pilots, flight crew, maintenance, and cargo ramp employers — American Airlines' largest hub — plus Norfolk Southern and CSX FRA rail yards, Duke Energy PHMSA gas and electric headquarters, and the Charlotte regional logistics corridor.
On-site DOT-compliant collections at Charlotte Douglas International Airport for FAA-regulated pilots, flight crew, maintenance, and cargo ramp employers — American Airlines' largest hub — plus Norfolk Southern and CSX FRA rail yards, Duke Energy PHMSA gas and electric headquarters, and the Charlotte regional logistics corridor. Charlotte anchors aviation — pilots, flight crew & air cargo (faa 14 cfr part 120), rail (fra), pipeline & utilities (phmsa) employer demand — including Charlotte Douglas CLT; CLT; Piedmont Airlines HQ; CLT cargo ramp, and related operators across North Carolina. For safety-sensitive CDL drivers, pipeline workers, railroad crews, defense contractors, and aviation employees who cannot leave a shift for a clinic visit, mobile collection keeps operations staffed while meeting federal custody requirements.
OnSite Employer Testing coordinates mobile drug testing collections at your Charlotte-area facility, yard, terminal, or job site. We do not operate a clinic. Review how it works or browse all locations we serve across the region.
Why Charlotte Employers Need Mobile Testing
CLT pilot and flight crew FAA 14 CFR Part 120 collections — pre-employment, random, post-accident, and return-to-duty for American Airlines, Piedmont Airlines, United, Delta, Southwest, Spirit, and regional carrier employer programs
CLT cargo ramp FAA safety-sensitive employer on-site collections for FedEx, UPS, DHL ground handling and cargo crews
program design and compliance obligations remain your responsibility under applicable law — we coordinate the collections
Industries We Serve in Charlotte
Charlotte Douglas CLT
Charlotte Douglas CLT — American Airlines largest hub FAA pilot/crew/maintenance safety-sensitive (FAA-regulated or employer-directed programs where applicable). We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Charlotte market.
CLT
CLT — United/Delta/Southwest/Spirit FAA pilot and flight crew employer programs (FAA-regulated or employer-directed programs where applicable). We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Charlotte market.
Piedmont Airlines HQ
Piedmont Airlines HQ — American Eagle regional carrier FAA pilot and crew testing (FAA-regulated or employer-directed programs where applicable). We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Charlotte market.
CLT cargo ramp
CLT cargo ramp — FedEx/UPS/DHL air cargo FAA safety-sensitive ground operations (FAA-regulated or employer-directed programs where applicable). We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Charlotte market.
Norfolk Southern Charlotte Terminal
Norfolk Southern Charlotte Terminal — FRA covered-service railroad employees (FRA-regulated or employer-directed programs where applicable). We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Charlotte market.
CSX Charlotte Yard
CSX Charlotte Yard — FRA covered railroad employees (FRA-regulated or employer-directed programs where applicable). We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Charlotte market.
Duke Energy Corporate HQ
Duke Energy Corporate HQ — PHMSA natural gas and electric transmission Charlotte metro (PHMSA-regulated or employer-directed programs where applicable). We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Charlotte market.
Piedmont Natural Gas (Duke Energy)
Piedmont Natural Gas (Duke Energy) — PHMSA gas distribution Carolinas (PHMSA-regulated or employer-directed programs where applicable). We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Charlotte market.
Amazon CLT1 / Charlotte fulfillment
Amazon CLT1 / Charlotte fulfillment — CDL delivery fleet FMCSA (FMCSA-regulated or employer-directed programs where applicable). We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Charlotte market.
NCDOT Division 10
NCDOT Division 10 — Charlotte metro highway construction contractor CDL fleets (FMCSA-regulated or employer-directed programs where applicable). We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Charlotte market.
Applicable Regulations
Employers in Charlotte commonly coordinate testing under:
- 14 CFR Part 120 — FAA anti-drug and alcohol misuse prevention for ALL aviation safety-sensitive employees: pilots (flight crewmembers), flight attendants, flight instructors, aircraft dispatchers, aircraft maintenance and preventive maintenance personnel, ground security coordinators, and air traffic controllers
- 49 CFR Part 40 — DOT collection and laboratory procedures
- 49 CFR Part 219 — FRA covered service Norfolk Southern and CSX Charlotte
- 49 CFR Part 199 — PHMSA Duke Energy and Piedmont Natural Gas pipeline operators
- 49 CFR Part 382 — FMCSA CDL logistics, construction, and distribution carrier fleets
- North Carolina Drug-Free Workplace Act (N.C.G.S. § 95-230 et seq.) — employer program requirements
Testing Services Available in Charlotte
We coordinate the following collections for Charlotte-area employers:
DOT drug testing collections (FMCSA, FRA, FAA, FTA, PHMSA)
- Pre-employment drug and alcohol testing
- Reasonable suspicion and return-to-duty testing
Breath alcohol testing (BAT certified)
- Oral fluid drug testing
Mobile and on-site collections at your facility, yard, or job site
On-Site Collections in Charlotte
Piedmont Airlines HQ on-site collections for regional carrier pilot and crew employer programs
Duke Energy and Piedmont Natural Gas PHMSA gas transmission and distribution field crew collections throughout the Carolinas
Norfolk Southern and CSX Charlotte FRA covered-service random and post-incident collections
NCDOT Division 10 highway construction CDL contractor testing along I-485, I-85, I-77, and I-277 corridors
24/7 post-accident and reasonable-suspicion response throughout the Charlotte metro
Use our instant quote calculator to scope your collection or visit our service areas page for broader coverage context.
Questions from employers in this region
CLT is American Airlines' largest hub — what FAA testing applies to pilots and crew based there?
14 CFR Part 120 requires FAA anti-drug and alcohol misuse prevention programs for ALL aviation safety-sensitive employees at air carriers operating under 14 CFR Parts 121, 135, and 145. At CLT this includes: pilots and flight crewmembers, flight attendants, aircraft dispatchers, aircraft maintenance and preventive maintenance personnel, ground security coordinators, and fuelers performing safety-sensitive functions. We coordinate FAA-compliant pre-employment, random, post-accident, reasonable suspicion, return-to-duty, and follow-up collections for airline and aviation employer programs at CLT.
Piedmont Airlines is headquartered in Charlotte — can you serve their pilot and crew employer program?
Yes. Piedmont Airlines HQ is in Charlotte and as a 14 CFR Part 121 air carrier, their pilot, flight attendant, dispatch, and maintenance employees are subject to FAA 14 CFR Part 120 testing requirements. We coordinate on-site FAA-compliant collections at Piedmont's Charlotte headquarters and CLT operations facilities.
Duke Energy is headquartered in Charlotte — what PHMSA testing applies to their gas utility workforce?
Duke Energy's Piedmont Natural Gas subsidiary operates one of the largest natural gas distribution systems in the Southeast. Covered-function pipeline employees — operators, maintenance workers, and emergency responders — are subject to PHMSA 49 CFR Part 199 anti-drug and alcohol misuse prevention requirements. We coordinate on-site PHMSA-compliant collections for Piedmont Natural Gas employees and contractors throughout the Carolinas from Charlotte HQ outward.
What does North Carolina's Drug-Free Workplace Act require for Charlotte employers?
North Carolina's Drug-Free Workplace Act (N.C.G.S. § 95-230 et seq.) establishes requirements for employer drug testing programs. For DOT-regulated employees, federal requirements preempt state law. For non-DOT employees, the NC DFWP Act sets standards for policy, testing procedures, and employee rights. We coordinate collections that satisfy both applicable federal DOT regulations and NC state program requirements.
Core programs: Mobile & on-site drug testing, DOT drug testing, Non-DOT drug testing, Post-accident testing, Reasonable suspicion testing, Random testing programs, Breath alcohol testing. Planning: how it works, instant quote calculator, all locations. Industries: employer industries we support. Long-form answers: Employer drug testing FAQ. Quick questions: Contact us.
For how we think about regions and feasibility, see service areas.
Nationally coordinated collection support
OnSite Employer Testing is headquartered in Illinois and coordinates mobile and on-site employer testing across selected regions and employer locations—including through partner collectors when your program, timing, and geography allow. Availability depends on location, timing, test type, site access, collector availability, and scheduling needs.
Scope collections for your Charlotte-area operation
Share your facility address, expected donor count, test reason, and DOT or non-DOT status — we confirm what is workable and send a written proposal.