DOT-COMPLIANT · ON-SITE EMPLOYER DRUG TESTING
Denver, Colorado Mobile Drug & Alcohol Testing
DEN Airport FAA · BNSF & Union Pacific FRA · Energy Sector · Amazon · I-70/I-25 FMCSA — Colorado marijuana law does NOT protect DOT workers.
Denver anchors the Rocky Mountain region's energy, logistics, and aviation economy. Denver International Airport (DEN) — the fifth-busiest in the U.S. — has United Airlines' second-largest hub and a sprawling cargo operation. BNSF and Union Pacific both maintain major Denver operations at the Globeville and North Yard classification facilities. The I-70/I-25 interchange is one of the most critical freight chokepoints in the interior West. Colorado Amendment 64 legalized recreational cannabis in 2012 — but every FMCSA, FAA, FRA, PHMSA, and FTA-regulated employee in Denver remains subject to 49 CFR Part 40, which prohibits marijuana use regardless of state law. OnSite Employer Testing dispatches certified collectors throughout the Denver Metro — airport ramps, rail yards, energy company offices, and warehouse docks.
OnSite Employer Testing coordinates mobile drug testing collections at your Denver-area facility, yard, terminal, or job site. We do not operate a clinic. Review how it works or browse all locations we serve across the region.
Denver: The Distribution Hub of the Rocky Mountain West
Denver anchors the Rocky Mountain region's energy, logistics, and aviation economy. Denver International Airport (DEN) — the fifth-busiest in the U.S. — has United Airlines' second-largest hub and a sprawling cargo operation. BNSF and Union Pacific both maintain major Denver operations at the Globeville and North Yard classification facilities. The I-70/I-25 interchange is one of the most critical freight chokepoints in the interior West. Colorado Amendment 64 legalized recreational cannabis in 2012 — but every FMCSA, FAA, FRA, PHMSA, and FTA-regulated employee in Denver remains subject to 49 CFR Part 40, which prohibits marijuana use regardless of state law. OnSite Employer Testing dispatches certified collectors throughout the Denver Metro — airport ramps, rail yards, energy company offices, and warehouse docks.
FAA 14 CFR Part 120 governs all safety-sensitive aviation employees at DEN. FRA 49 CFR Part 219 covers BNSF and Union Pacific crews at Denver's major yards. FTA 49 CFR Part 655 applies to RTD safety-sensitive employees. FMCSA 49 CFR Part 382 governs CDL holders throughout Denver Metro. PHMSA 49 CFR Part 199 applies to pipeline workers. Colorado Amendment 64 legalized recreational marijuana but 49 CFR Part 40.151(e) prohibits MROs from canceling a DOT positive due to any state law — marijuana positives disqualify DOT safety-sensitive employees.
Program design and compliance obligations remain your responsibility under applicable law — we coordinate the collections.
Industries Served in Denver
Aviation
Aviation — Denver International Airport (DEN), United hub, cargo operators. We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Denver market.
Railroad
Railroad — BNSF Globeville Yard, Union Pacific North Yard. We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Denver market.
Energy
Energy — Extraction Oil & Gas, DCP Midstream, Bonanza Creek, Denver Basin operators. We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Denver market.
CDL freight
CDL freight — I-70/I-25 corridor, transcontinental and regional carriers. We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Denver market.
Transit
Transit — RTD light rail, bus, and commuter operations. We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Denver market.
E-commerce and fulfillment
E-commerce and fulfillment — Amazon, Chewy, Denver metro DCs. We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Denver market.
Construction
Construction — Denver metro urban renewal and I-70 corridor projects. We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Denver market.
Healthcare
Healthcare — UCHealth, SCL Health, Children's Hospital Colorado. We coordinate on-site collections for pre-employment, random, post-accident, and reasonable suspicion events at employer facilities throughout the Denver market.
Applicable RegulationsDenver, CO
Employers in Denver commonly coordinate testing under:
- FAA 14 CFR Part 120 — Pilots, flight attendants, dispatchers, A&P mechanics, ground security coordinators, and ramp personnel at Denver International Airport (DEN) — United, Southwest, Frontier, and cargo operators
- FRA 49 CFR Part 219 — BNSF and Union Pacific engineers, conductors, dispatchers, and MOW employees at Denver Globeville, North Yard, and Colorado & Southern subdivisions
- FMCSA 49 CFR Part 382 — CDL drivers on I-70, I-25, I-76, and I-270 serving Denver's dense distribution and energy logistics corridor
- PHMSA 49 CFR Part 199 — Pipeline workers for Denver metro gas distribution, DCP Midstream, and Colorado's extensive natural gas gathering infrastructure
- FTA 49 CFR Part 655 — RTD (Regional Transportation District) operators, maintenance personnel, and safety-sensitive employees
- DOT Return-to-Duty & Follow-Up — SAP-supervised employees across all modes
Testing Services Available in Denver
We coordinate the following collections for Denver-area employers:
DOT drug testing collections (FMCSA, FRA, FAA, FTA, PHMSA)
- Pre-employment drug and alcohol testing
- Reasonable suspicion and return-to-duty testing
Breath alcohol testing (BAT certified)
- Oral fluid drug testing
Mobile and on-site collections at your facility, yard, or job site
On-Site Collections in Denver
Pre-employment, Random, Reasonable Suspicion, Post-Accident — urine, oral fluid, and EBT
Colorado Marijuana Compliance — Amendment 64 does NOT exempt DOT-regulated workers from 49 CFR Part 40
Energy sector non-DOT panels — oil and gas companies, midstream operators, pipeline contractors
Hair follicle and instant-result screening for rapid construction and fulfillment hiring
Use our instant quote calculator to scope your collection or visit our service areas page for broader coverage context.
Questions from employers in this region
Colorado legalized marijuana — does that affect DOT testing for our drivers and pilots?
No. Colorado Amendment 64 has zero effect on federal DOT testing under 49 CFR Part 40. Marijuana positives are still violations for FMCSA, FAA, FRA, PHMSA, and FTA-regulated employees. MROs cannot cancel a DOT positive based on state law.
Can you handle FAA testing for DEN-based aviation crews?
Yes. We enroll safety-sensitive aviation employees at DEN into FAA 14 CFR Part 120 compliant random pools and coordinate unannounced collections with appropriate airport access.
Do you serve BNSF and Union Pacific employees at Denver's rail yards?
Yes. FRA 49 CFR Part 219 post-accident, reasonable suspicion, and random collections are available for BNSF Globeville and Union Pacific North Yard employees.
Do you cover RTD's transit workforce under FTA 49 CFR Part 655?
Yes. We coordinate FTA-compliant collections for RTD safety-sensitive employees — operators, maintenance, and dispatch personnel.
Core programs: Mobile & on-site drug testing, DOT drug testing, Non-DOT drug testing, Post-accident testing, Reasonable suspicion testing, Random testing programs, Breath alcohol testing. Planning: how it works, instant quote calculator, all locations. Industries: employer industries we support. Long-form answers: Employer drug testing FAQ. Quick questions: Contact us.
For how we think about regions and feasibility, see service areas.
Nationally coordinated collection support
OnSite Employer Testing is headquartered in Illinois and coordinates mobile and on-site employer testing across selected regions and employer locations—including through partner collectors when your program, timing, and geography allow. Availability depends on location, timing, test type, site access, collector availability, and scheduling needs.
Mobile Testing for Denver's Most Complex Multi-Modal Employer Base
DEN airport, BNSF/UP rail yards, RTD, energy sector, and I-70/I-25 carriers — one call dispatches certified collections to your Denver site.